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           Products  Fluorescence Spectrophotometers  F-7100
 

 

 


Fluorescence Spectrophotometer F-7100

Research grade, high sensitivity fluorescence spectrophotometer. The Hitachi F7100 features the highest sensitivity in its class and an extremely long lifetime light source as well as high speed scanning and a host of accessories.

 

  
   
FL33 ETHANOL MEASUREMENT AND ENZYME ASSAY WITH USE OF AUTOMATIC 4-TURRET ACCESSORY

The F-7000 fluorescence spectrophotometer achieves a high sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min) wavelength scan at the highest level in its class, with a wide dynamic range up to 6 digits. Furthermore, it is applied to research, development, and quality control in a broad range of fields, such as industrial materials, life sciences, and biotechnology.
The 4-turret accessory used in this experiment automatically measures, by computer control, the time course of the fluorescence intensity at regular time intervals through 4 rotating cells installed in a turret. A reaction trace for an enzyme is used to measure the time course of fluorescence intensity in plural samples. Previously, the time course was measured for one sample at a time, so the measurement time required was proportional to the number of samples. Application of the automatic 4-turret accessory reduces measurement time.
An enzyme assay was applied to ethanol measurement in this experiment. The amount of ethanol in the fish is increased according to a decline of the freshness, so this measurement is referred to as the freshness index. Ethanol is usually measured with a spectrophotometer. When ethanol is present in trace amounts,
a low concentration measurement is performed with a fluorescence spectrophotometer. In this experiment, the general enzyme assay reagent "F-kit ethanol (J.K.International)" was used. In addition to the enzyme assay, a spectrophotometer was used to measure ethanol amounts. The analysis conditions were optimized, and an analytical curve for a standard solution was created.
   
FL32 FLUORESCENCE SPECTRUM MEASUREMENT METHOD ALLOWING A COMPARISON OF DIFFERENT EQUIPMENT - SPECTRAL CALIBRATION OF INSTRUMENTAL RESPONSE AND VALIDATION METHOD, AND MEASUREMENT EXAMPLE FOR A STANDARD FLUORESCENT MATERIAL

Keywords: Fluorescence spectrum, Spectrum calibration, Instrumental response, Rhodamine B, Quinine sulfate, Standard material, GLP/GMP program
 
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